Highlights
Experimental design and sequence analysis report You work at a biological testing facility and have been asked to look into the following three problems:
Part 1
A new compound SK234 has been identified and purified from a common soil-based fungus, which may have potential as a new anticancer drug to treat breast cancer. You have been tasked with investigating this problem and to design a series of experiments to test this theory.
a) Identify five appropriate scientific questions based on this information and design a suitable hypothesis for testing one of these questions.
b) Design an experimental strategy to test your hypothesis. Indicate sufficient detail so that this can be fully critiqued by your boss before you will be allowed to begin work in this area. You will need to identify suitable controls, any sources of potential experimental error, sample sizes, any potential confounding variables and how you plan to analyse and present the data at the end of this process. Your experimental strategy should be justified using appropriate literature.
Part 2
In your second case, a whole family have been referred to you for immediate genetic analyses.
A male patient was on holiday with his family and he has now developed a fever and anaemia. A range of diagnostic genetic tests using a sample of his blood have been conducted using polymerase chain reaction (PCR) and DNA sequencing to determine the potential cause. All the tests come back as normal apart from one, in the battery of pathogen detection tests, an unusual result arose.
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